MultiFun, a cellfunction assignment schema.

Gretta Serres and Monica Riley, July 14, 2004.

Some of the categories have been assigned by Nicole Perna et al. at ASAP (http://asap.ahabs.wisc.edu/annotation/php/ASAP1.htm).

categories with changes/additions in this edition:

3.1.3.7     binding, sequestering

4.S.195   2-O-alpha-mannosyl-D-glycerate

1.1.1.26  2-O-alpha-mannosyl-D-glycerate degradation

4.S.196   Zn(2+)

1 Metabolism

            1.1 Carbon compound utilization

                        1.1.1 Carbohydrates/Carbon compounds

                                   1.1.1.1 D-allose catabolism

                                   1.1.1.2 2,5-ketogluconate metabolism

                                   1.1.1.3 D-arabinose catabolism

                                   1.1.1.4 D-galactarate catabolism

                                   1.1.1.5 D- galacturonate catabolism

                                   1.1.1.6 D-glucarate catabolism

                                   1.1.1.7 D-glucuronate catabolism

                                   1.1.1.8 L-arabinose catabolism

                                   1.1.1.9 L-idonate catabolism

                                   1.1.1.10 L-lyxose metabolism

                                   1.1.1.11 Fucose catabolism

                                   1.1.1.12 Galactitol catabolism

                                   1.1.1.13 Galactonate catabolism

                                   1.1.1.14 Lactose degradation

                                   1.1.1.15 Mannose catabolism

                                   1.1.1.16 Rhamnose catabolism

                                   1.1.1.17 Sorbitol degradation

                                   1.1.1.18 Trehalose degradation, low osmolarity+

                                   1.1.1.19 Xylose catabolism

                                   1.1.1.20 Glycol degradation

                                   1.1.1.21 Mannitol degradation

                                   1.1.1.22 Ribose degradation

                                   1.1.1.23 Galactose degradation

                                   1.1.1.24 Sorbose degradation

                                   1.1.1.25 L-ascorbate degradation

                                   1.1.1.26 2-O-alpha-mannosyl-D-glycerate degradation

                        1.1.2 Fatty acids (fatty acid oxidation)

                                   1.1.2.1 Degradation of short-chain fatty acids

                                   1.1.2.2 3-phenylpropionate and 3-(3-hydroxyphenyl)propionate degradation

                                   1.1.2.3 Propionate degradation

                                   1.1.2.4 Anaerobic fatty acid oxidation pathway

                        1.1.3 Amino acids

                                   1.1.3.1 L-alanine degradation

                                   1.1.3.2 L-serine degradation

                                   1.1.3.3 Arginine catabolism

                                   1.1.3.4 Glutamate degradation

                                   1.1.3.5 Glycine cleavage

                                   1.1.3.6 Proline utilization

                                   1.1.3.7 Threonine catabolism

                                   1.1.3.8 Tryptophan utilization

                                   1.1.3.9 L-cysteine catabolism

                                   1.1.3.10 Lysine cleavage

                                   1.1.3.11 Histidine degradation

                                   1.1.3.12 Leucine degradation

                                   1.1.3.13 Methionine degradation

                                   1.1.3.14 Valine degradation

                                   1.1.3.15 Phenylalanine, tyrosine degradation

                        1.1.4 Amines

                                   1.1.4.1 Phenylethylamine degradation

                                   1.1.4.2 Carnitine degradation

                                   1.1.4.3 Ornithine degradation

                        1.1.5 Others

                                   1.1.5.1 Phenylacetic acid degradation

                                   1.1.5.2 Ethanol degradation

                                   1.1.5.3 Eugenol catabolism

                                   1.1.5.4 Beta-ketoadipate pathway

                                   1.1.5.5 Mandelate catabolism

            1.2 Macromolecule degradation

                        1.2.1 RNA

                        1.2.2 DNA

                        1.2.3 Proteins/peptides/glycopeptides

                        1.2.4 Polysaccharides

                                   1.2.4.1 Glycogen catabolism

                                   1.2.4.2 Chitin catabolism

            1.3 Energy metabolism (carbon)

                        1.3.1 Glycolysis

                        1.3.2 Pentose phosphate shunt, oxidative branch

                        1.3.3 Pyruvate dehydrogenase

                        1.3.4 Tricarboxylic acid cycle

                        1.3.5 Fermentation

                        1.3.6 Aerobic respiration

                        1.3.7 Anaerobic respiration

                        1.3.8 ATP proton motive force interconversion

                        1.3.9 Entner-Doudoroff pathway

            1.4 Energy production/transport

                        1.4.1 Electron donor

                        1.4.2 Electron acceptor

                        1.4.3 Electron carrier

            1.5 Building block biosynthesis

                        1.5.1 Amino acids

                                   1.5.1.1 Glutamate

                                   1.5.1.2 Glutamine

                                   1.5.1.3 Arginine

                                   1.5.1.4 Proline

                                   1.5.1.5 Aspartate

                                   1.5.1.6 Asparagine

                                   1.5.1.7 Lysine, diaminopimelate

                                   1.5.1.8 Threonine

                                   1.5.1.9 Methionine

                                   1.5.1.10 Glycine

                                   1.5.1.11 Serine

                                   1.5.1.12 Cysteine

                                   1.5.1.13 Phenylalanine

                                   1.5.1.14 Tyrosine

                                   1.5.1.15 Tryptophan

                                   1.5.1.16 Histidine

                                   1.5.1.17 Alanine

                                   1.5.1.18 Isoleucine/valine

                                   1.5.1.19 Leucine

                                   1.5.1.20 Chorismate

                                   1.5.1.21 Homoserine

                        1.5.2 Nucleotide

                                   1.5.2.1 Purine biosynthesis

                                   1.5.2.2 Pyrimidine biosynthesis

                                   1.5.2.3 Purine ribonucleotide biosynthesis

                                   1.5.2.4 Pyrimidine ribonucleotide/ribonucleoside biosynthesis

                        1.5.3 Cofactor, small molecule carrier

                                   1.5.3.1 Biotin

                                   1.5.3.2 Folic acid

                                   1.5.3.3 Lipoate

                                   1.5.3.4 Molybdenum (molybdopterin)

                                   1.5.3.5 Coenzyme A

                                   1.5.3.6 Pyridoxine (vitamin B6)

                                   1.5.3.7 Nicotinamide adenine dinucleotide (NAD)

                                   1.5.3.8 Thiamine (Vitamin B1)

                                   1.5.3.9 Riboflavin (Vitamin B2), FAD, FMN

                                   1.5.3.10 Glutathione

                                   1.5.3.11 Menaquinone (MK), ubiquinone (Q)

                                   1.5.3.12 Heme, porphyrin

                                   1.5.3.13 Cobalamin (Vitamin B12)

                                   1.5.3.14 Enterochelin (enterobactin)

                                   1.5.3.15 Chrysobactin

                                   1.5.3.16 Achromobactin

                                   1.5.3.19 Isoprenoid biosynthesis

                                   1.5.3.20 Tetrahydrobiopterin biosynthesis

                                   1.5.3.21 Pyochelin biosynthesis

                        1.5.4 Fatty acid and phosphatidic acid

            1.6 Macromolecules (cellular constituent)  biosynthesis

                        1.6.1 Phospholipid

                        1.6.2 Colanic acid (M antigen)

                        1.6.3 Lipopolysaccharide

                                   1.6.3.1 O antigen

                                   1.6.3.2 Core region

                                   1.6.3.3 Lipid A

                        1.6.4 Enterobacterial common antigen  (surface glycolipid)

                        1.6.5 K antigen

                        1.6.6 Osmoregulated periplasmic glucan

                        1.6.7 Peptidoglycan (murein)

                        1.6.9 Polysaccharides, cytoplasmic

                        1.6.10 Lipoprotein

                        1.6.11 Glycoprotein

                        1.6.12 Flagella

                        1.6.13 Fimbria, pili, curli

                        1.6.15 Large molecule carriers

                                   1.6.15.1 Cytochromes

                                   1.6.15.2 Thioredoxin, glutaredoxin

                                   1.6.15.3 Biotin carboxyl carrier protein

                                   1.6.15.4 Acyl carrier protein

                        1.6.16 Cellulose biosynthesis

            1.7 Central intermediary metabolism

                        1.7.1 Unassigned reversible reactions

                        1.7.2 Glyoxylate bypass

                        1.7.3 Pentose phosphate shunt, non-oxidative branch

                        1.7.6 Glycerol metabolism

                        1.7.7 Galactose metabolism

                        1.7.8 Gluconeogenesis

                        1.7.9 Misc. glucose metabolism

                        1.7.10 Sugar nucleotide biosynthesis, conversions

                        1.7.12 Amino sugar conversions

                        1.7.13 Amino acid conversion

                        1.7.14 Polyamine biosynthesis

                        1.7.15 2'-deoxyribonucleotide/ribonucleoside metabolism

                        1.7.17 Formyl-tetrahydrofolate biosynthesis

                        1.7.18 Betaine biosynthesis

                        1.7.19 Incorporation of metal ions

                        1.7.20 S-adenosyl methionine biosynthesis

                        1.7.21 Glyoxylate degradation

                        1.7.22 Carnitine metabolism

                        1.7.23 Methylglyoxal metabolism

                        1.7.24 Cyanate catabolism

                        1.7.25 Glycolate metabolism

                        1.7.26 Allantoin assimilation

                        1.7.27 Pyridoxal 5'-phosphate salvage

                        1.7.28 Pyruvate catabolism

                        1.7.29 Acetate catabolism

                        1.7.31 Aminobutyrate catabolism

                        1.7.32 Putrescine catabolism

                        1.7.33 Nucleotide and nucleoside conversions

                        1.7.34 Peptidoglycan (murein) turnover, recycling

                        1.7.35 Lactate oxidation

                        1.7.36 Trehalose biosynthesis

                        1.7.37 C1 assimilation, serine pathway

                        1.7.38 Methionine salvage pathway

            1.8 Metabolism of other compounds

                        1.8.1 Phosphorous metabolism

                        1.8.2 Sulfur metabolism

                        1.8.3 Nitrogen metabolism

2 Information transfer

            2.1 DNA related

                        2.1.1 DNA replication

                        2.1.2 DNA restriction/modification

                        2.1.3 DNA recombination

                        2.1.4 DNA repair

                        2.1.5 DNA degradation

            2.2 RNA related

                        2.2.2 Transcription related

                        2.2.3 RNA modification

                        2.2.4 RNA degradation

                        2.2.5 tRNA

                        2.2.6 rRNA, stable RNA

                        2.2.7 Antisense RNA

            2.3 Protein related

                        2.3.1 Amino acid-activation

                        2.3.2 Translation

                        2.3.3 Posttranslational modification

                        2.3.4 Chaperoning, folding

                        2.3.5 Export, signal peptide cleavage

                        2.3.6 Turnover, degradation

                        2.3.7 Nucleoproteins, basic proteins

                        2.3.8 Ribosomal proteins

                        2.3.9 Non-ribosomal peptide synthetase

3 Regulation

            3.1. Type of regulation

                        3.1.1 DNA structure level

                                   3.1.1.1 DNA bending, supercoiling, inversion

                                   3.1.1.2 Methylation

                        3.1.2 Transcriptional level

                                   3.1.2.1 Sigma factors, anti-sigmafactors

                                   3.1.2.2 Activator

                                   3.1.2.3 Repressor

                                   3.1.2.4 Complex regulation

                                               3.1.2.4.1         More than one signal needed

                                               3.1.2.4.2 Regulons or multilayer component regulatory systems

                                               3.1.2.4.3 Two-component regulatory systems (external signal)

                                               3.1.2.4.4 Quorum sensing

                                   3.1.2.5 Action unknown

                        3.1.3 Posttranscriptional

                                   3.1.3.1 Translation attenuation and efficiency

                                   3.1.3.2 Covalent modification, demodification, maturation

                                   3.1.3.3 Inhibition / activation of enzymes

                                   3.1.3.4 Proteases, cleavage of compounds

                                   3.1.3.5 Multilayer regulatory systems

                                   3.1.3.6 Antisense RNA

                                   3.1.3.7 binding, sequestering

                        3.1.4 Regulation level unknown

            3.3 Genetic unit regulated

                        3.3.1 Operon (regulation of one operon)

                        3.3.2 Regulon (a network of operons encoding related functions)

                        3.3.3 Stimulon (ie. environmental stimulus)

                        3.3.4 Global

            3.4 Trigger (some information added)

            3.5 Trigger modulation (some information added)

4  Transport

            4.1 Channel-type Transporters

                        4.1.A. alpha-type channels

                                   4.1.A.1. The Voltage-gated Ion Channel (VIC) Superfamily

                                   4.1.A.8. The Major Intrinsic Protein (MIP) Family

                                   4.1.A.11 The Chloride Channel (ClC) Family

                                   4.1.B.17 The Outer Membrane Factor (OMF) Family

                                   4.1.A.22 The Large Conductance Mechanosensitive Ion Channel (MscL) Family

                                   4.1.A.23 The Small Conductance Mechanosensitive Ion Channel (MscS) Family

                                    4.1.A.46  The H+- or Na+-translocating Bacterial Flagellar Motor (Mot) Family

                        4.1.B Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily)

                                   4.1.B.1 The General Bacterial Porin (GBP) Family

                                   4.1.B.3 The Sugar Porin (SP) Family

                                   4.1.B.9 The FadL Outer Membrane Protein (FadL) Family

                                   4.1.B.10 The Nucleoside-specific Channel-forming Outer Membrane Porin (Tsx) Family

                                   4.1.B.14 The Outer Membrane Receptor (OMR) Family

                                   4.1.B.21 The OmpG Porin (OmpG) Family

            4.2  Electrochemical potential driven transporters

                        4.2.A. Porters (Uni-, Sym- and Antiporters)

                                   4.2.A.1 The Major Facilitator Superfamily (MFS)

                                   4.2.A.2 The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family

                                   4.2.A.3 The Amino Acid-Polyamine-Choline (APC) Family

                                   4.2.A.4 The Cation Diffusion Facilitator (CDF) Family

                                   4.2.A.6 The Resistance-Nodulation-Cell Division (RND) Superfamily

                                   4.2.A.7 The Drug/Metabolite Transporter (DMT) Superfamily

                                   4.2.A.8 The Gluconate:H+ Symporter (GntP) Family

                                   4.2.A.9 The Cytochrome oxidase biogenesis (Oxa1) Family

                                   4.2.A.10 The 2-Keto-3-Deoxygluconate Transporter (KDGT) Family

                                   4.2.A.13 The C4-Dicarboxylate Uptake (Dcu) Family

                                   4.2.A.14 The Lactate Permease (LctP) Family

                                   4.2.A.15 The Betaine/Carnitine/Choline Transporter (BCCT) Family

                                   4.2.A.16 The Telurite-resistance/Dicarboxylate Transporter (TDT) Family

                                   4.2.A.17 The Proton-dependent Oligopeptide Transporter (POT) Family

                                   4.2.A.19 The Ca2+:Cation Antiporter (CaCA) Family

                                   4.2.A.20 The Inorganic Phosphate Transporter (PiT) Family

                                   4.2.A.21 The Solute:Sodium Symporter (SSS) Family

                                   4.2.A.23 The Dicarboxylate/Amino Acid:Cation (Na+ or H+) Symporter (DAACS) Family

                                   4.2.A.25 The Alanine/Glycine:Cation symporter (AGCS) Family    

                                   4.2.A.26 The Branched Chain Amino Acid:Cation Symporter (LIVCS) Family

                                   4.2.A.27 The Glutamate:Na+ Symporter (GltS) Family

                                   4.2.A.33 The NhaA Na+:H+ Antiporter (NhaA) Family

                                   4.2.A.34 The NhaB Na+:H+ Antiporter (NhaB) Family

                                   4.2.A.36 The Monovalent Cation:Proton Antiporter-1 (CPA1) Family

                                   4.2.A.37 The Monovalent Cation:Proton Antiporter-2 (CPA2) Family

                                   4.2.A.38 The K+ Transporter (Trk) Family

                                   4.2.A.39 The Nucleobase:Cation Symporter-1 (NCS1) Family

                                   4.2.A.40 The Nucleobase:Cation Symporter-2 (NCS2) Family

                                   4.2.A.41 The Concentrative Nucleoside Transporter (CNT) Family

                                   4.2.A.42 The Hydroxy/Aromatic Amino Acid Permease (HAAAP) Family

                                   4.2.A.44 The Formate-Nitrite Transporter (FNT) Family

                                   4.2.A.45 The Metal Ion Transporter (MIT) Family

                                   4.2.A.47 The Divalent Anion:Na+ Symporter (DASS) Family

                                   4.2.A.49 The Ammonium Transporter (Amt) Family

                                   4.2.A.53 The Sulfate Permease (SulP) Family

                                   4.2.A.55 The Manganese (Nramp) Fam.

                                   4.2.A.58 The Phosphate:Na+ Symporter (PNaS) Family

                                   4.2.A.61 The C4-dicarboxylate Uptake C (DcuC) Family

                                   4.2.A.64 The Type V Secretory Pathway or Twin Arginine Targeting (Tat) Family

                                   4.2.A.66 The Multi Antimicrobial Extrusion (MATE) Family

                                   4.2.A.68 The p-Aminobenzoyl-glutamate Transporter (AbgT) Family

                                   4.2.A.69 The Auxin Efflux Carrier (AEC) Family

                                   4.2.A.72 The K+ uptake permease (KUP) family

                                   4.2.A.75 The L-lysine exporter (LysE) family

                                   4.2.A.76 The Resistance to Homoserine/Threonine (RhtB) Family

                        4.2.C Ion-gradient driven energizers

                                   4.2.C.1 TonB Family of Auxiliary Proteins for Energization of OMR-mediated Transport

            4.3 Primary Active Transporters

                        4.3.A. Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters

                                   4.3.A.1 The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases

                                               4.3.A.1.a ATP binding component

                                               4.3.A.1.m membrane component

                                               4.3.A.1.p periplasmic binding component

                                               4.3.A.1.am ATP binding and membrane component

                                   4.3.A.2 The H+/Na+-translocating F-, V- and A-type ATPase (F-ATPase) Superfamily

                                   4.3.A.3 The P-type ATPase (P-ATPase) Superfamily

                                   4.3.A.4 The Arsenite-Antimonite (Ars) Efflux Family

                                   4.3.A.5 The Type II (General) Secretory Pathway (IISP) Family

                                   4.3.A.6 The Type III (Virulence-related) Secretory Pathway (IIISP) Family

                                   4.3.A.7 The Type IV (Conjugal DNA-Protein Transfer) Secretory Pathway (IVSP) Family

                        4.3.D. Oxidoreduction-driven Active Transporters

                                   4.3.D.1 The Proton- or sodium ion-translocating NADH Dehydrogenase (NDH) Family

                        4.3.D.4 The Proton-translocating Cytochrome Oxidase (COX) Superfamily

            4.4 Group Translocators

                        4.4.A Phosphotransferase Systems (PEP-dependent PTS)

                                   4.4.A.1 The PTS Glucose-Glucoside (Glc) Family

                                   4.4.A.2 The PTS Fructose-Mannitol (Fru) Family

                                   4.4.A.3 The PTS Lactose-N,N'-Diacetylchitobiose-betaucoside (Lac) Family

                                   4.4.A.4 The PTS Glucitol (Gut) Family

                                   4.4.A.5 The PTS Galactitol (Gat) Family

                                   4.4.A.6 The PTS Mannose-Fructose-Sorbose (Man) Family

            4.8.A Accessory Factors Involved in Transport

                        4.8.A.1 The Membrane Fusion Protein (MFP) Family

                        4.8.A.2 The Secretin Auxiliary Lipoprotein (SAL) Family

                        4.8.A.3 MPA1 Family auxillary transport protein

                        4.8.A.7 The Phosphotransferase System Enzyme I (EI) Family

                        4.8.A.8 The Phosphotransferase System HPr (HPr) Family

            4.9.A Transporters of Unknown Classification

                        4.9.A Recognized transporters of unknown biochemical mechanism

                                   4.9.A.1 The Polysaccharide Transporter (PST) Family

                                   4.9.A.4 The Nicotinamide Mononucleotide (NMN) Uptake Permease (PnuC) Family

                                   4.9.A.8 The Ferrous Iron Uptake (FeoB) Family

                                   4.9.A.13 The Short Chain Fatty Acid Transporter (scFAT) Family

                                   4.9.A.16 The Septal DNA Translocator (SDT) Family

                                   4.9.A.17 The Metal Ion Transporter (MIT) Family

                        4.9.B Putative uncharacterized transport protein

                                   4.9.B.3 The Putative Bacterial Murein Precursor Exporter (MPE) Family

                                   4.9.B.4  The Putative Efflux Transporter (PET) Family

                                   4.9.B.6 The Toxic Hok/Gef Protein (Hok/Gef) Family

                                   4.9.B.10 The 6TMS Putative MarC Transporter (MarC) Family

                                   4.9.B.18 The SecDF-associated Single Transmembrane Protein (SSTP) Family

                                   4.9.B.21 The Frataxin (Frataxin) Family

                                   4.9.B.22 The Putative Permease (PerM) Family

                                   4.9.B.24 The Testis-Enhanced Gene Transfer (TEGT) Family

                                   4.9.B.25 The YbbM (YbbM) Family

                                   4.9.B.27 The YdjX-Z (YdjX-Z) Family

                                   4.9.B.28 The YqaE (YqaE) Family

                                   4.9.B.29 The YebN (YebN) Family

                                   4.9.B.31 The YqiH (YqiH) Family